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. 2023 Apr 3;24(7):6685. doi: 10.3390/ijms24076685

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effects of miR-6-3p, miR-9a-3p, miR-9d-3p, miR-11-3p, and miR-13a-3p on development of T. castaneum. (A,B) The expression level of miR-6-3p, miR-9a-3p, miR-9d-3p, miR-11-3p, and miR-13a-3p in wild-type (WT) insects, negative controls (NC) and the corresponding miRNA-specific antagomirs treated-samples (AI) at 48 h post injection by PCR. (C) Phenotypes induced by injecting miRNA-specific antagomirs. Three individuals from each group were used to determine gene expression level by PCR, and the results were calculated from three biological samples. “*” indicates p < 0.05. Scale bar: 1000 μm.