Figure 1.

Flow chart for computer-assisted measurements of skin layer thickness: (a) H&E-stained whole skin section, showing individual layer thickness: epidermis (blue), dermis (purple), hypodermis (green), and Region of Interest (ROI, white rectangle) used to survey each full-length layer. (b) Epidermis isolation within the ROI converted into a Traced Area (TA, orange). (c) Equation used to calculate layer thickness (FB, Fiber Breadth) using measured perimeter of the isolated layer (PR, μm) and TA (μm²). (d) Resulting conversion of the irregular fiber (b) into a rectangle measuring the average thickness of the layer while preserving its original length-to-width ratio.