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. 2023 Apr 6;24(7):6865. doi: 10.3390/ijms24076865

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterization of HFD testis and sperm parameters. (A,B) H&E staining of testis (A) and cauda SPZ (B) collected from CTRL (n = 6) and HFD (n = 6) mice. (A) In testis sections, germ cell lumen infiltration was indicated by black arrowheads; scale bar: 100 μm. (B) Anomalous sperm heads were indicated by black arrowheads; scale bar: 50 μm; scale bar inset: 10 μm. (C) Percentage of anomalous sperm heads in CTRL and HFD cauda SPZ; data were reported as the percentage of anomalous sperm heads/total SPZ. Sperm viability (D) and motility (E) assay in CTRL and HFD cauda SPZ; data were expressed as the percentage of nonviable/total SPZ and motile/live SPZ, respectively, and reported as mean ± SEM; ** p < 0.01.