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. 2023 Apr 6;24(7):6865. doi: 10.3390/ijms24076865

Table 3.

Primer sequences and annealing temperatures.

Gene Primers Sequences 5′-3′ Tm (°C)
Mmu circ-MEMO1 S ACTATGATGAATCCCAGGGGG 52
Mmu circ-MEMO1 AS CAGGGGCACATGATGGGAAG
Mmu circ-DNAH7 S TACACGGGCCCTGCATTGTA 57
Mmu circ-DNAH7 AS AGGAGAGACCCAGCATGTGTA
Mmu circ-MAPT S GTCAGGTCGAAGATTGGCTCT 55
Mmu circ-MAPT AS ATACTGGTTCAAAGCCTTGCC
Mmu circ-DNER S TGTGTCCTAGACCCATGCAG 53
Mmu circ-DNER AS TCTGCAACAAACTTCCAGACAC
Mmu circ-CPSF6 S TCGTTAGAAGATTTGCCCTTGT 52
Mmu circ-CPSF6 AS ACAACAGGACTCTGACCATGA
Mmu circ-PTPN11 S TACGGGGTCATGCGTGTTAG 53
Mmu circ-PTPN11 AS GGGGTGAAACCATTTGTCCG
Mmu circ-ADAM10 S CCTATGTCTTCACAGACCGGG 55
Mmu circ-ADAM10 AS TGGGGATAGTCTGAAGGTGC
Mmu circ-RESP18 S TCTCCCCAAAAGATGGTCAGG 53
Mmu circ-RESP18 AS TGCCTTCGGGTACAATCTGG
Mmu circ-TULP4 S ATAAACTTCAACCTGCGAGGC 53
Mmu circ-TULP4 AS TCCCGGTTAATTCAGGAGCCA
Mmu circ-MAGED1 S TGGCTTCTTCTGGGTTGAGTAAAT 52
Mmu circ-MAGED1 AS AGTAAAGGCCTGGGGAAACCTG
Mmu circ-CLNS1A S ACTGTTGCCGGACAGTTTGA 53
Mmu circ-CLNS1A AS CAGGACAGGCGGTGTATAGT
Mmu circ-INPP5B S CCTCCTGCCAGTGAGAAGTG 58
Mmu circ-INPP5B AS TCAGCTGAGTGATGTTCTTTCCT
Mmu circ-SMAD2 S TGACTACACCCACTCCATTCC 56
Mmu circ-SMAD2 AS TCAGAGCAAGTGCTGGGATGT
Mmu circ-KDM4D S AAACTTGGGGCTTGAAGCGT 54
Mmu circ-KDM4D AS GGGGTGCAGCAGAATCTCTT
Mmu circ-NFAT5 S AAAAGAGCACTCGTGCCAGA 52
Mmu circ-NFAT5 AS TCAGAGAATTGCATAAAATGGGG
Mmu circ-QRICH1 S TGGGAATGTAAGCAGCTTGGG 56
Mmu circ-QRICH AS
Mmu circ-ATRN S
Mmu circ-ATRN AS
Mmu circ-PCSK6 S
Mmu circ-PCSK6 AS
TGACAATCCACCTGAGGCT
GCGTATGACCTGACTTCTAGGG
TGAGCATCCAGGACCTTATGT
GGAGTGGACTCTGGAAGTGC
ACCTTGGCGACCCTTGTTTC
56
58
RPS18 S GAGACTCTGGATGCTAACTAG 56
RPS18 AS GGACATCTAAGGGCATCACAG
Cyclophilin-A S TGGTCTTTGGGAAGGTGAAAG 52
Cyclophilin-A AS TGTCCACAGTCGGAAATGGT
PLAG1 S CCATACACATGACCCCAACA 58
PLAG1 AS CTTCACACCCCCAGATGACT