Figure 2.

Effect of 10-min treatment with positive controls (histamine 100 μM and ionomycin 5 μM) and a negative control (BAPTA-AM 50 μM 30-min pre-treatment) on (A) ATP and (B) Glu release from the astrocyte cultures. The effect of BAPTA-AM was tested in the presence and absence of ionomycin. The bars represent the mean estimates, whereas the whiskers indicate standard errors of means. Dashed lines indicate the level of ATP and Glu released in control conditions (fresh experimental medium or HEPES buffered Ca²⁺-containing solution, respectively). The number of biological replicates n ≥ 9 for each condition. One-way analysis of variance with post hoc Dunnett’s test, significance level α = 0.05. * p < 0.05, ** p < 0.01, *** p < 0.001. ns—not significant, Hist—histamine, B—BAPTA-AM, Ion—ionomycin, Ion + B—ionomycin following 30-min pre-incubation with BAPTA-AM.