Figure 3.
Effects of BUT and IPA on ATP and Glu release from the cultured astrocytes. (A) Effect on ATP release following 10-min treatment. (B) Effect on Glu release following 10-min treatment. (C) Attenuation of the BUT effect on ATP release by 30-min pre-incubation with BAPTA-AM 50 μM. (D) Effect on ATP release following 24-h pre-treatment with IPA. (E) Effect on Glu release following 24-h pre-treatment with IPA. The bars represent the mean estimates, whereas the whiskers indicate standard errors of means. Dashed lines indicate the levels of ATP and Glu release in control conditions (fresh experimental medium or HEPES buffered Ca2+-containing solution, respectively). Number of biological replicates n ≥ 9 for each condition. One-way analysis of variance with post hoc Dunnett’s test, significance level α = 0.05. ** p < 0.01, *** p < 0.001. ns—not significant, B—BAPTA-AM.