Table 2.
Genes significantly upregulated by H2O2 in HepG2 senescence.
| Gene | Task | Fold Change | UPla | ULu |
|---|---|---|---|---|
| CDKN1A/P21 | Cyclin-dependent kinase inhibitor 1A | 3.39 | Yes * | Yes |
| MMP3 | Senescence marker, SASP | 5.26 | Yes | Yes |
| PTEN | Tumor suppressor gene | 1.39 | Yes | Yes |
| RB1 | Tumor suppressor gene, signaling pathway | 1.57 | Yes | Yes |
| CDKN1B/p27 | Cyclin-dependent kinase inhibitor 1B | 1.11 | Yes | Yes |
| NFκB1 | Signaling pathway | 1.13 | Yes | Yes |
| MAPK14 | Signaling pathway | 1.68 | Yes | Yes |
| AKT1 | Signaling pathway | 1.11 | Yes | Yes |
| LONP1 | Mitochondrial protein | 1.05 | Yes | Yes |
| SDHA | Mitochondrial protein | 2.64 | Yes | Yes |
| SDHC | Mitochondrial protein | 1.24 | Yes | Yes |
| PDHA1 | Mitochondrial protein | 1.66 | Yes | Yes |
| ITPR2 | Calcium channel | 1.21 | Yes | Yes |
| TNF | Secreted cytokines, SASP | 2.35 | No | No |
| TGFB1 | Transforming growth factor beta 1, SASP | 1.81 | Yes | Yes |
| IGF2 | Insulin-like growth factor | 1.50 | Yes | Yes |
| BMI1 | Polycomb ring finger oncogene | 1.14 | Yes | Yes |
| VCAN/CSPG2 | Induction greater than 4-fold in HPEC senescence [41] | 1.63 | No | No |
| MMP2 | SASP, induction greater than 4-fold in HPEC senescence [41] | 1.37 | No | No |
| DAO | Induction greater than 4-fold in HPEC senescence [41] | 1.69 | Yes | Yes |
| PRODH | Identified in senescence depending on p53 [42] | 2.77 | Yes | Yes |
| SLC52A1/GPR172B | Identified in senescence depending on p53 [42] | 3.32 | Yes | Yes |
Gene expression was normalized to HMBS expression. Fold change is the ratio of expression in senescent cells to the expression in the control groups. * Yes: Upla or Ulu modulated H2O2-caused gene expression alterations. No: UPla or ULu did not affect gene expression changes.