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. 2023 Apr 6;24(7):6808. doi: 10.3390/ijms24076808

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Verification of proteomic data by immunofluorescence analyses. The secretory proteins thrombospondin-4 (THBS4) and biglycan identified by proteomic analysis (left) as well as the proteins SEC63, BET1, ERGIC-3, and Golgin-97 associated with ER and (transport to) Golgi (right) were stained in p1, p3, p5, and p7 as well as in age-matched patients with neurogenic muscular atrophy (NMA) and normal disease controls (NDC). Co-staining with spectrin (red) was performed to visualize the sarcolemma. Scale bars 50 µm.