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. 2023 Mar 30;24(7):6515. doi: 10.3390/ijms24076515

Figure 1.

Figure 1

gRNAs targeting the genic and intergenic region of the BCTIV genome. Genic regions encoding coat protein (CP—referred to as V1), movement protein (MP—referred to as V1), ss/ds DNA regulating protein (ssDNA—referred to as V3), and Replication protein (REPA and B—referred to as C1 and C2) were targeted via CRISPR/Cas9 system with the designed gRNAs. Short intragenic region (referred to as SIR) and long intragenic region (referred to as LIR) were also represented in the figure. Detailed information about the gRNA is provided in Supplementary File S1.