Figure 5.

RT-qPCR validation of candidate genes identified via RNA-seq or implicated in previous tinnitus studies. VS tissue from patients without tinnitus (white bars, circles) was compared to VS tissue from patients with tinnitus (gray bar, triangles) (n = 19 total samples; see Table S1 for patient information). While there was a trend for lower expression of several genes in VS tissue associated with tinnitus, this trend did not meet our criterion for significance. Error bars indicate standard deviations. Abbreviations: RNA-seq, RNA sequencing; RT-qPCR, reverse transcription–quantitative polymerase chain reaction.