Table 2.
HPLC experimental conditions for the determination of some classes of phenolic compounds.
| Compounds | Stationary Phase | Column Temperature (°C) |
Mobile Phase | Flow Rate of the Mobile Phase (mL/min) | Wavelength (nm) | References |
|---|---|---|---|---|---|---|
| Phenolic acids | C18 (4.6 mm × 150 mm, 5 μm) | 25 | (A) 0.1% formic acid (B) 100% methanol |
0.7 | 270 | [90] |
| Phenols | C18 (2.1 mm × 150 mm, 5 μm) | 30 | (A) H2O (0.1% v/v HFor) (B) MeOH 100% (0.1% v/v HAcO) Gradient elution |
0.5 | 320, 280 | [87] |
| Polyphenols Flavonoids Phenolic acids | C18 (4.6 mm × 150 mm, 5 μm) | - | (A) Methanol (B) Acetic acid:water (1:99, v/v) |
1 | 210–400 | [91] |
| Anthocyanins Polyphenols |
Superspher 100 RP, (250 × 4.6 mm 18.5 μm) | 30 | (A) 10% formic acid in water (B) Methanol:water:formic acid (45:45:10, v/v/v) |
0.8 | 530 | [92] |
| Anthocyanins | C18 | 20 | (A) Water:formic acid:ACN (87:10:3, v/v/v) (B) Water:formic acid:CAN (40:10:50, v/v/v) |
1 | 520 | [93] |
| Polyphenols | C18 (4.6 mm × 250 mm, 5 μm) | 40 | (A) water containing 0.5% v/v formic acid (B) acetonitrile:water (6:4) containing 0.5% v/v formic acid Gradient elution |
1 | 220–360 | [50] |
| Flavonols | Polar-RP 80A (150 mm × 4.6 mm 5 μm) | 30 | (A) water with 0.1% formic acid (B) methanol with 0.1% formic acid Gradient elution |
0.8 | [94] |