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. 2023 Apr 4;28(7):3205. doi: 10.3390/molecules28073205

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In vitro catalytic activity of recombinant CtF3′5′H1-encoding enzyme using HPLC-MS analysis. (A) HPLC analysis of different flavonoid substrates catalyzed by recombinant CtF3′5′H1-encoding enzyme. The intensity of product changes by CtF3′5′H1 was observed with the increase of substrate concentration. (B) The oxidation capacity of naringenin to eriodictyol catalyzed by recombinant CtF3′5′H1. (C) Time-course study of the enzymatic reaction, and LC/MS analysis of the substrate (naringenin) and product (eriodictyol). One-way ANOVA was used to calculate statistical significance among different groups.