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. 2023 Apr 12;14:2057. doi: 10.1038/s41467-023-37632-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Total GCase activity in mouse cortical neurons as a measure of cellular uptake after 2 h of treatment with imiglucerase, mGCase or mGCase-mBS. Data was normalised to Gba + /+ cells. n = 3 (for mGCase and mGCase-mBS, the two lowest concentrations have only been repeated twice). b Total GCase activity in H4 cells as a measure of cellular uptake after 2 h of treatment with imiglucerase, hGCase-hBS-NB or hGCase-hBS. Data was normalised to GBA + / + cells. n = 3. c Live imaging of GCase activity (LysoFQ-GBA) and lysosomes (SiR lyso) and quantification of colocalising signal in mouse cortical neurons. Data was normalised to Gba + /+ cells. n = 3 (4–9 fields analysed per plate). d Live imaging of GCase activity (LysoFQ-GBA) and lysosomes (SiR lyso) and quantification of colocalising signal in H4 cells. Data was normalised to GBA + / + cells. n = 3 (8–16 fields analysed per plate). Bar graphs represent group means + SEM. Each data point represents an independent measurement. Activity data were analysed by two-way ANOVA (Tukey’s multiple comparisons test). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. n = number of independent measurements. Source data are provided as a Source Data file.