Fig. 1. DAXX promotes lipogenic gene expression and its SUMO-binding property is required.

a Validation of shRNA-mediated DAXX knockdown (KD) and the overexpression of wild-type DAXX (WT OE) and the SUMO-binding defective mutant (DSM OE) compared to cells with a control vector (CTL) in MDA-MB-231 cells by immunoblotting. b An expression heatmap of selected genes in the lipogenesis pathway in MDA-MB-231-derived cells (CTL, KD, wt OE, and DSM OE) based on microarray data (triplicates for each group). c, d Ingenuity pathway analysis (IPA) for upstream regulators (c) and canonical pathways (d) using differentially expressed genes in KD, WT, and DSM OE cells compared to CTL cells based on the mRNA microarray data as in (b). P values were obtained via the right-tailed Fisher’s exact test implemented in the Ingenuity Pathway Analysis software in (d). e RT-qPCR analysis of the indicated genes in the MDA-MB-231-derived cells. mRNA level was normalized against that of ACTB. Data are shown as mean ± SEM (n = 3 biologically independent samples). P values are based on unpaired, two-tailed t test vs CTL. GGPP Geranylgeranyl diphosphate, RA rheumatoid arthritis. Source data are provided as a Source Data file.