Fig. 3. DAXX promotes lipid production.

a, d Impact of DAXX expression levels on acetate-dependent de novo lipid synthesis using [¹⁴C]-acetate labeling in the absence of serum in cell lines derived from MDA-MB-231 (a) and MDA-MB-468 (d) (n = 3 biologically independent samples). b, e Principal component analysis (PCA) of lipidome in the panel of four MDA-MB-231 and MDA-MB-468-derived cell lines (CTL, KD, WT OE, and DSM OE). Levels of cholesterol and diacylglycerols as detected by LC/MS in the indicated cell lines are shown. n refers to the number of lipid molecules in the indicated lipid classes. c Validation of shRNA-mediated DAXX knockdown (KD) and the overexpression of wild-type DAXX (WT OE) and the SUMO-binding defective mutant (DSM OE) compared to cells with a control vector (CTL) in MDA-MB-468 cells by immunoblotting. f Lipogenesis pathways activated by DAXX. Representative genes that are downregulated by DAXX KD but upregulated by WT DAXX OE are shown in blue. Identified metabolites by LC/MS that positively correlate with DAXX expression levels are denoted. All bar graph data are presented as mean values ± SEM. n.s.: not significant (P > 0.05, unpaired two-tailed t test). Source data are provided as a Source Data file.