Fig. 7. The DAXX–SREBP interaction is critical for lipid synthesis and tumor growth.

a Relative mRNA levels of DAXX in MDA-MB-231 cells expressing the WT or del 327–335 mutant cDNA of DAXX as determined by RT-qPCR (n = 2). b DAXX protein levels in control cells and those with KD, WT and del 327–335 mutant cDNA of DAXX. c The DAXX del 327–337 mutant impaired de novo lipogenesis. Serum-starved cells were labeled with [1-¹⁴C] acetate and total lipids were isolated. Radioactivity was counted and normalized against total protein level. The P value was calculated using ordinary one-way ANOVA (n = 3 biologically independent samples). d PCA of lipidomes in MDA-MB-231 cells expressing the del 327–335 mutant and WT DAXX. Each dot represents a sample (n = 4). e Hierarchical clustering heatmap analysis of global lipidomes in cells expressing the del 327–335 mutant and WT DAXX. f–i Heatmap analysis of lipid molecules that were highly differentially expressed between MDA-MB-231 cells with the del 327–335 mutant and WT DAXX (f, glycerolipids, g, glycerophospholipids, h, fatty acids, and i, sterols). j MDA-MB-231 cells expressing the del 327–335 mutant and WT DAXX were xenografted into mammary fat pads of female NSG mice (n = 4 tumor-bearing mice). Representative images of dissected tumors are shown. The final tumor weights are plotted. Data are presented as mean values ± SEM and the P values were calculated based on unpaired two-tailed t test. k A cartoon depicting the importance of DAXX–SREBP interaction for lipid production and tumorigenesis. Source data are provided as a Source Data file.