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. 2023 Apr 12;14:1927. doi: 10.1038/s41467-023-37501-0

Table 1.

PCR primers used for this study

RT-qPCR primers (5’ to 3’)
ActinB-F-Real GCTCCTCCTGAGCGCAAGTACTC
ActinB-R-Real GTGGACAGCGAGGCCAGGAT
Daxx-RT-F GAGGCGTCTCTCCTCACAAC
Daxx-RT-R TCTCATGCACTGACCTTTGC
SREBP1-F CTGCTGTCCACAAAAGCAAA
SREBP1-R GGTCAGTGTGTCCTCCACCT
SREBP2-F ATCGCTCCTCCATCAATGAC
SREBP2-R TTCCTCAGAACGCCAGACTT
FASN-F CACAGGGACAACCTGGAGTT
FASN-R ACTCCACAGGTGGGAACAAG
HMGCR-F GTCATTCCAGCCAAGGTTGT
HMGCR-R CATGGCAGAGCCCACTAAAT
STARD4-F GGCGAGTTGCTAAGAAAACG
STARD4-R CCCTGGGCGTATATGGTCTA
HMGCS1-F GGGACACATATGCAACATGC
HMGCS1-R CACTGGGCATGGATCTTTTT
FDPS-RT-F CCAAGAAAAGCAGGATTTCG
FDPS-RT-R CCGGTTATACTTGCCTCCAA
ChIP primers
FASN-prom-F1 TAGAGGGAGCCAGAGAGACG
FASN-prom-R1 GCTGCTCGTACCTGGTGAG
ACACA-prom-F1 CAAGGGAAATTGAGGCTGAG
ACACA-prom-R1 CGTTCCAGGAGCATCTGATT
FASN-ChIP-3’UTR-F1 GTGAACCATGACTGCACCAC
FASN-ChIP-3’UTR-R1 GAGCCCTCGGTGACATACAT
ACACA-ChIP-3’UTR-F1 TCATGGCCAAACTGTTGAAA
ACACA-ChIP-3’UTR-R1 TGGGGTCCATTGTTTCTGAT
SREBF-prom-F2 TCCTTTAAACAAGGCGGAGA
SREBF-prom-R2 TCAGCAGCTCAGATTTGCAT
Primers for amplifying a fragment in the SREBF2 promoter
SREBF2-prom-F2 CAGCTGAAGCTTGCATGCCTGCAGGTAGGCAGCTGGGAAGATGA
SREBF2-prom-R2 GAGTATATATAGGACTGGGGATCCGTGAGGGTCTCCATGGTCTC