Table 2.
Antibodies used in this study
| Antibody target | Vendor/source | Vendor catalog # | Dilution |
|---|---|---|---|
| DAXX (for IB) | Bethyl Laboratories | A301-352A | 1:20,000 (IB) |
| DAXX (for IB) | Bethyl Laboratories | A301-353A | 1:20,000 (IB) |
| DAXX (for IP, IB, IF PLA, and ChIP) | The Developmental Studies Hybridoma Bank | PCRP-DAXX-5G11 | Hybridoma supernatant. 1:100 for IB, 1:5 for IF, and 1:2 for PLA |
| DAXX (for IP and IB) | GenScript, rabbit polyclonal | This study | 1:10,000 for IB |
| FASN (for IB and IF) | ProteinTech | 10624-2-AP | 1:20,000 for IB, and 1:700 for IF |
| FASN | Santa Cruz | SC-55580 | 1:20,000 (IB) |
| ACC1 | Cell Signaling Technology | 3676, clone C83B10 | 1:20,000 (IB) |
| ACLY | Cell Signaling Technology | 13390, clone D1X6P | 1:10,000 (IB) |
| ACSS2 | Cell Signaling Technology | 3658, clone D19C6 | 1:20,000 (IB) |
| SREBP2 | Cayman Chemical | 10007663 | 1:10,000 for IB, 1:300 for IF and 1:100 for PLA |
| SREBP2 | BD Biosciences | 557037, clone IgG-1C6 | 1:1000 for IB |
| SREBP1 | Santa Cruz | SC-13551, clone 2A4 | 1:500 (IB) |
| SREBP1 | ProteinTech | 14088-1-AP | 1:3000 for IB, 1:300 for IF and 1:100 for PLA |
| FLAG | Cell Signaling Technology | 14793 | 1:10,000 (IB) |
| FLAG (IB, IP and ChIP) | Millipore-Sigma | F1804, clone M2 | 1:1000 (IB), 2 µg per IP or ChIP experiment |
| GFP | Cell Signaling Technology | 2956 | 1:3000 (IB) |
| PCNA | Epitomics | 2714-1, clone EPR3821 | 1:20,000 (IB) |
| Alpha-tubulin | Millipore-Sigma | T5168, clone B-5-1–2 | 1:50,000 (IB) |
| HSP60 | BD Transduction Laboratories | H99020 | 1:50,000 (IB) |
| Rabbit IgG HRP-linked antibody | Cell Signaling Technology | 7074 | 1:10,000 (IB) |
| Mouse IgG HRP-linked antibody | Cell Signaling Technology | 7076 | 1:10,000 (IB) |
| Normal mouse IgG (for IP/ChIP control) | Santa Cruz | SC-2025 | 2 µg per IP or ChIP experiment |