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. 2023 Apr 12;14:2066. doi: 10.1038/s41467-023-37589-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Representative FACS plots showing expression of UEA1 and Ly51 by total EpCAM1⁺ cells, K19^-EpCAM1⁺ cells and K19⁺EpCAM1⁺ cells at E12.5, and corresponding quantitation, n = 12, from 3 independent experiments. b Krt19^CreERTtdTom embryos were fate-mapped via tamoxifen administration at E12.5, and the neonatal thymus was harvested at birth. Fate-mapped cells were quantitated (n = 11, from 3 independent experiments) c Immunofluorescence of fate-mapped thymi at PNd0, K5 (turquoise) K19-tdTom (red), Scale bar denotes 20um. Image representative of 4 thymi, from 3 independent experiments. d Expression of Ly51 and UEA1 by total EpCAM1⁺ cells, K19-Tom^- EpCAM1⁺ cells (non-fate-mapped), and K19-tdTom⁺EpCAM1⁺ cells (fate-mapped) at PNd0, bar charts show corresponding quantitation, n = 11, from 3 independent experiments. e Representative FACS plots illustrating the phenotype of fate-mapped mTEC subsets within PNd0 fate-mapped thymus. mTEC^hi (MHCII^hiCD80^hi, n = 11), mTEC^lo (MHCII^loCD80^lo, n = 11), Aire⁺ (MHCII^hiCD80^hiAire⁺, n = 11), CCL21⁺ (n = 6) and tuft cells (MHCII^loCD80^loDCLK1⁺, n = 5) and corresponding quantitation. f Immunofluorescence of fate-mapped thymi at PNd0, Aire (turquoise) K19-tdTom (red), EpCAM1 (blue). Scale bar,10 µm. Image representative of 4 thymi. Data analysed using a Student’s t test. The data are shown as mean ± SEM.