Table 3.
Immune protection of live vaccine with fructose in M. salmoides.
| Group | Immunogen | Total fish | Survive No | ADR(%) | RPS1 (%) | RPS2 (%) |
|---|---|---|---|---|---|---|
| 1 | Live vaccine | 60 | 33 | 45.0% | 55.0% | |
| 2 | Live vaccine with 0.18mg Fructose | 60 | 37 | 38.3% | 61.7% | 14.8% |
| 3 | Live bacteria with 1.8mg Fructose | 60 | 46 | 23.3% | 76.7% * | 48.2%* |
| 4 | PBS | 60 | 0 | 100.0% | ||
| 5 | 1.8mg Fructose | 60 | 0 | 100.0% |
All the treatment was same volume (0.1mL). Survived, the number of fish survived in the experiment. ADR, accumulating death rates; RPS, relative percent survival. RPS was calculated as RPS1 = 1 - (% mortality of Fructose and/or bacteria treated group/% mortality of control group) × 100. RPS2 = 1 - (% mortality of Fructose and bacteria treated group/% mortality of bacteria treated group) × 100. The experiments are repeated twice. * P < 0.05.