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. 2023 Apr 10;222(5):e202106022. doi: 10.1083/jcb.202106022

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© 2023 Moore et al.

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Figure 5. — RFWD3 promotes the reversal of stalled replication forks. (A and B) Representative images of normal (A) and reversed (B) replication forks detected by electron microscopy (EM) in control or RFWD3-depleted U2OS cells upon HU treatment. (C) Representative images of DNA replication bubbles detected by EM in HU-treated control and RFWD3-depleted U2OS cells. (D) Detection of RFWD3 levels in U2OS cells transfected with siRFWD3-4 for the experiment in Fig. 5 E. See Fig. S3 C for the depletion efficiency of other replicates used in Fig. 5 E. (E) Percentage of reversed replication forks detected by EM in control and RFWD3-depleted U2OS cells upon HU treatment (2 mM for 5 h). The mean and SEM from three independent experiments are shown (**P < 0.01, t test, see Fig. S3 C). The number of replication intermediates analyzed for each condition is indicated in parentheses.