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. 2023 Mar 30;17:1148292. doi: 10.3389/fnbeh.2023.1148292

FIGURE 2.

FIGURE 2

Prior stress is necessary to promote icv CRF reduction of 5-HT in the CeA. (A) In naïve, unstressed rats, treated icv with saline (N = 4), or rat/human CRF (CRF) treatment (3 or 10 μg) have no significant (no *) effect on the release of serotonin (5-HT; mean ± SEM) in the CeA over time (data are normalized to the 3 samples prior to CRF treatment at 0 min, arrow). The CRF groups were not statistically different from the saline group (condition: p > 0.29, condition x time: p > 0.35; GLM-TWRMANOVA). (B) In stressed rats (1 h of restraint stress 24 h prior to infusion), icv CRF significantly [for 3 (N = 6) and 10 μg (N = 8)] reduces 5-HT release in the CeA of rats, compared with CRF alone [*GLM-TWRMANOVA, condition, p < 0.0018 panel (A)], samples taken prior to CRF treatment (–40, –20, 0 min; time, *p < 0.001), or saline controls (N = 4; condition x time, *p < 0.001) that also received prior stress. In animals exposed to prior stress, 5-HT responses in the CeA to the 3 μg and 10 μg doses of CRF did not differ significantly.