FIGURE 4.

Without stress, a combination of icv CRF and AVP, is necessary to promote reduction of 5-HT in the CeA. While either CRF or AVP combined with prior stress (Figures 2B, 3B) produce significant reductions of 5-HT release in CeA, in the absence of prior stress, icv AVP plus CRF (N = 8) produce synergistic effects resulting in a dramatic and persistent decline in extracellular 5-HT in the CeA (*time: < 0.0001; condition x time: p < 0.0001). This decline is significant compared with samples taken prior to CRF treatment (time: –40, –20, 0 min), or saline controls (N = 7; condition x time) that also received no prior stress.