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. 2023 Mar 30;14:1149339. doi: 10.3389/fimmu.2023.1149339

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Copyright © 2023 Hu, Huang, Zheng, Zhang, Zeng, Nie, Pang and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-199a-5p positively regulates the proliferation and osteogenic differentiation of hSCAPs in vitro. (A) The CCK8 assay. (B) The Crystal violet staining and quantification assay. (C) ALP staining of different groups after 3 and 7 days of osteogenic induction (scale bar = 400 μm). (D) ALP activity assay. (E) The relative mRNA expression level of osteogenic marker genes RUNX2, OSX, ALP, and OCN. (F) ARs staining assay (scale bar = 400 μm). (G) Semi-quantitative analysis of ARs stained images. (H) The relative mRNA expression level of osteogenic marker genes RUNX2, OSX, ALP, and OCN. The data were shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.