Figure 2.

Internal morphology, seed coat scarification and testa thickness of C. album dimorphic seeds. (A) Cross-section of dry black seed and visible endosperm protrusion after TR. (B) Images and box-and-whisker plot (min-max) of testa thickness of black and brown seed. Mean ± SEM values are presented of at least 50 seeds of each seed type. (C) Microscopic cross-section highlighting tissue features of C. album seeds after having undergone methyl methylacrylate Technovit fixation embedding protocol stained in safranin and counter stained in methylene blue. (D) Maximum germination (mean ± SEM values) of seedlot #1 dormant black, after-ripened black and mechanically scarified dormant black seeds.