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. 2022 Nov 23;237(2):576–584. doi: 10.1111/nph.18566

Table 1.

Characteristics of substrates used in the ingrowth mesh bags in the cafeteria experiment.

Substrate N deposition (kg ha−1 yr−1) At collection After 17 months preincubation
pH pH Mineralised N (μg g−1 OM) OM (%) C/N EMF DNA (%)
Organic, central Sweden 5 4.2 4.4 2540 63 26 5.6
Mull soil, central Sweden 5 6.1 4.9 3220 8 16 4.5
Organic, southern Sweden 10–15 4.1 4.9 2090 66 25 0.6
P‐fertilised organic, southern Sweden 10–15 3.6 4.9 1340 71 29 3.1
Sand 0 0
Sand with 1% apatite 0 0

The soil was collected from mature Picea abies forests in two areas with contrasting atmospheric N deposition levels. pH was measured at the time of collection and after 17 months of preincubation. Mineralised N was calculated as the difference in inorganic N between un‐sieved, wet soils and sieved, dried soils after preincubation. Organic matter (OM) % and C/N were measured after preincubation. The proportion of ectomycorrhizal fungal (EMF) DNA was acquired through sequencing of ITS2 markers.