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. 2022 Nov 24;47(2):367–373. doi: 10.1002/cbin.11966

Figure 4.

Figure 4

PC12 (Riken) cells in uncoated versus collagen IV‐coated flask. PC12 (Riken) cells were adapted from Dulbecco's modified eagle's medium (DMEM) to Roswell Park Memorial Institute (RPMI) (both in 15% horse serum and 2.5% foetal bovine serum) as described in the Section 2. Cells were seeded into an uncoated T25 flask and a 10 µg/mL collagen IV‐coated flask. Representative phase contrast images are shown after three passages in the RPMI media and imaged after 2–3 days of seeding. (a) PC12 (Riken) cells in uncoated T25 flask containing RPMI media are rounded, phase‐bright and clumped. (b) PC12 (Riken) cells in collagen IV‐coated T25 flask in the same RPMI media are spread out, phase dark and attached well. Scale bar 200 µM.