Scheme 2.

Introduction of NodJ leads to five possible parallel series of nodulisporic acids and NodX further functionalises 1‐series compounds to produce 2‐series nodulisporic acids. NAD₃ or downstream products in this series were not identified in this study. Proposed mechanistic details can be found in the Supp. Info. (Figure S60). The configuration of C2′′ of NAD₆ was assigned based on the previously established configuration of this position for NAA₁. ^[3d]