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The Holo prodrug is reduced by GSH. Titration of Holo with GSH; Holo is reduced at 5 mM GSH. The assay was performed by adding the specified amount of GSH to 50 μM Holo in 75 mM Tris‐HCl, pH 7.4 (in presence of air). The spectrum of quantitatively reduced Holo (with TCEP) is included for comparison.
