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Physical and biochemical properties of hPL per method. The freeze‐thawing (black bars) and high calcium (15 mM Ca2+ without glass, white bars) methods were compared with the 5 mM Ca2+ with glass method (shaded bars) for (A) transmittance, (B) fibrinogen concentration, (C) PDGF‐AB concentration, and (D) hADSC doubling time (h). Data are shown as mean with SD (n = 4), *p < .05 and **p < .01.
