. 2023 Mar 13;14(3):284. doi: 10.3390/insects14030284

Table 1.

Primer sequences for PCR.

Gene	Primer (Forward/Reverse)	Sequence (Forward and Reverse) 5′ → 3′	PCR Conditions (Annealing)	References
COI	F 2183	CAACATTTATTTTGATTTTTTGG	50 °C	Monteiro & Pierce, 2001 [13]
COI	R 3014	TCCAATGCACTAATCTGCCATATTA	50 °C	Monteiro & Pierce, 2001 [13]
Cytb	F revcb2h	TGAGGACAAATATCATTTTGAGGW	50 °C	Simmons et al., 2001 [14]
Cytb	R rebcbj	TCAGGTCGAGCTCCAATTCATGT	50 °C	Simmons et al., 2001 [14]
16S	F 13398	CGCCTGTTTATCAAAAACAT	50 °C	Simon et al., 1994 [15]
16S	R 12887	CCGGTCTGAACTCAGATCAT	50 °C	Simon et al., 1994 [15]
28S-D2	F 3665	AGAGAGAGTTCAAGAGTACGTG	58 °C	Belshaw et al., 1997 [16]
28S-D2	R 4068	TTGGTCCGTGTTTCAAGACGGG	58 °C	Belshaw et al., 1997 [16]