FIG 2.

Comparison of the ROD.7 and 35922ec ICEs. Illustrated is an alignment of the 47-kb 35922ec extrachromosomal element in the strain MGCS35922 genome with the ROD.7 equivalent elements of all seven isolates of the cohort. Shown in a gradient of gray, indicating percent sequence identity, are strain-to-strain aligned regions. These elements have conjugal transfer gene content indicating that they are ICEs. All have a PinE superfamily site-specific serine integrase at their 3′ end which directs integration into the 23S rRNA methyltransferase gene rmlD (aka rumA). The 5′ end of rmlD is part of the 35922ec ICE. These ICEs are most closely related among the six stG62647 strains, being 100% identical in sequence among strains MGCS35823, MGCS36044, MGCS36083, and MGCS36089 throughout their 51.1-kbp lengths, indicating a very recent common ancestor. The ICE in stC74a strain MGGS36055 has 52.6% of its sequence in common with that of strain MGCS36044 ROD.7. Most distant is the 35922ec ICE, which has 45.9% of its sequence in common with that of strain MGCS36044 ROD.7.