TABLE 1.
Performances of BacT/Alert or Bactec blood culture bottles for the Candida species detection in the absence or presence of antifungal agentsa
| Organism and condition used | BacT/Alert FAP bottles |
Bactec PAF bottles |
Bactec MICF bottles |
|||
|---|---|---|---|---|---|---|
| Recovery, % (no. of bottles detected as positive/no. of bottles tested) | Mean time (h) to detection | Recovery, % (no. of bottles detected as positive/no. of bottles tested) | Mean time (h) to detection | Recovery, % (no. of bottles detected as positive/no. of bottles tested) | Mean time (h) to detection | |
| Candida species grown without antifungal agents | ||||||
| C. auris | 100 (10/10) | 21.4 | 100 (10/10) | 21.2 | 100 (10/10) | 20.9 |
| C. albicans | 100 (14/14) | 21.5 | 100 (14/14) | 23.3 | 100 (14/14) | 19.9 |
| C. glabrata | 100 (14/14) | 17.8 | 100 (14/14) | 31.5 | 100 (14/14) | 17.5 |
| C. krusei | 100 (12/12) | 21.5 | 100 (12/12) | 27.1 | 100 (12/12) | 22.2 |
| C. parapsilosis | 100 (14/14) | 36.9 | 100 (14/14) | 31.5 | 100 (14/14) | 32.8 |
| C. tropicalis | 100 (14/14) | 20.5 | 100 (14/14) | 19.8 | 100 (14/14) | 19.3 |
| All species | 100 (78/78) | 23.4 | 100 (78/78) | 25.9 | 100 (78/78) | 22.1 |
| Candida species grown with echinocandins | ||||||
| C. auris | 100 (18/18) | 23.1 | 100 (18/18) | 34.1 | 100 (18/18) | 70.1 |
| C. albicans | 55.6 (10/18) | 40.0 | 33.3 (6/18) | 74.5 | 0 (0/18) | – |
| C. glabrata | 100 (18/18) | 18.0 | 100 (18/18) | 41.6 | 100 (18/18) | 20.5 |
| C. krusei | 88.9 (16/18) | 22.8 | 44.5 (8/18) | 42.6 | 0 (0/18) | – |
| C. parapsilosis | 100 (18/18) | 40.1 | 100 (18/18) | 34.8 | 100 (18/18) | 39.8 |
| C. tropicalis | 77.8 (14/18) | 26.8 | 44.5 (8/18) | 46.8 | 0 (0/18) | – |
| All species | 87.0 (94/108) | 27.7 | 70.4 (76/108) | 41.5 | 50.0 (54/108) | 43.5 |
| Candida species grown with azoles | ||||||
| C. auris | 100 (12/12) | 21.9 | 100 (12/12) | 22.7 | 100 (12/12) | 36.7 |
| C. albicans | 100 (18/18) | 22.1 | 100 (18/18) | 28.8 | 33.3 (6/18) | 97.8 |
| C. glabrata | 100 (18/18) | 19.1 | 100 (18/18) | 34.0 | 100 (18/18) | 20.4 |
| C. krusei | 100 (12/12) | 22.7 | 100 (12/12) | 29.8 | 83.3 (10/12) | 51.6 |
| C. parapsilosis | 100 (18/18) | 38.4 | 100 (18/18) | 33.1 | 33.3 (6/18) | 39.1 |
| C. tropicalis | 100 (18/18) | 21.9 | 100 (18/18) | 21.2 | 100 (18/18) | 41.9 |
| All species | 100 (96/96) | 24.6 | 100 (96/96) | 28.7 | 72.9 (70/96) | 41.4 |
| Candida species grown with amphotericin Bb | ||||||
| C. albicans | 100 (6/6) | 22.9 | 100 (6/6) | 39.4 | 66.6 (4/6) | 51.0 |
| C. glabrata | 100 (6/6) | 18.6 | 100 (6/6) | 44.9 | 0 (0/6) | – |
| C. krusei | 100 (6/6) | 24.3 | 100 (6/6) | 31.7 | 66.6 (4/6) | 35.2 |
| C. parapsilosis | 100 (6/6) | 38.3 | 100 (6/6) | 42.9 | 66.6 (4/6) | 50.1 |
| C. tropicalis | 100 (6/6) | 24.5 | 100 (6/6) | 30.0 | 66.6 (4/6) | 49.7 |
| All species | 100 (30/30) | 25.7 | 100 (30/30) | 35.8 | 53.3 (16/30) | 46.5 |
Growth of Candida species without or with antifungal agents (i.e., echinocandins, azoles, or amphotericin B) was detected after incubating the indicated blood culture bottles in the BacT/Alert VIRTUO or the Bactec FX blood culture automated system, respectively. In the case of recovery, the times to detection were recorded for each of Candida species allowed to grow in the absence or presence of antifungals agents. The symbol – indicates no recovery.
The list of Candida species allowed to grow in the presence of amphotericin B does not include C. auris because of a MIC value of 4 μg/mL to the polyene antifungal drug (see Table S1) that led us to consider the organism resistant to amphotericin B, as already noted (3).