TABLE 3.

Recovery and time to detection of Candida species from BacT/Alert or Bactec blood culture bottles according to clinically relevant azole antifungal concentrations^a

Antifungal drug and peak serum-level concn (%) usedb		Organism used	BacT/Alert FAP bottles		Bactec PAF bottles		Bactec MICF bottles
			Recovery (no. of bottles detected as positive/no. of bottles tested)	Mean time (h) to detection	Recovery (no. of bottles detected as positive/no. of bottles tested)	Mean time (h) to detection	Recovery (no. of bottles detected as positive/no. of bottles tested)	Mean time (h) to detection
Fluconazole	100	C. albicans	2/2	23.4	2/2	28.2	0/2	–
	50		2/2	21.8	2/2	24.8	0/2	–
	25		2/2	21.1	2/2	24.7	0/2	–
	No drug		2/2	20.9	2/2	22.7	2/2	20.1
	100	C. glabrata	2/2	19.7	2/2	36.7	2/2	21.4
	50		2/2	18.6	2/2	34.9	2/2	18.5
	25		2/2	19.0	2/2	34.0	2/2	18.1
	No drug		2/2	17.9	2/2	31.7	2/2	17.2
	100	C. parapsilosis	2/2	40.3	2/2	32.3	2/2	50.8
	50		2/2	39.3	2/2	30.3	2/2	33.5
	25		2/2	38.2	2/2	30.1	2/2	33.2
	No drug		2/2	38.2	2/2	30.1	2/2	32.9
	100	C. tropicalis	2/2	21.0	2/2	20.4	2/2	40.2
	50		2/2	20.3	2/2	20.7	2/2	29.4
	25		2/2	20.2	2/2	20.5	2/2	28.7
	No drug		2/2	19.7	2/2	20.1	2/2	18.2
Posaconazole	100	C. auris	2/2	22.4	2/2	25.6	2/2	74.3
	50		2/2	22.8	2/2	24.7	2/2	50.0
	25		2/2	21.7	2/2	23.2	2/2	26.4
	No drug		2/2	22.4	2/2	22.1	2/2	21.1
	100	C. albicans	2/2	23.4	2/2	41.8	0/2	–
	50		2/2	21.2	2/2	36.7	0/2	–
	25		2/2	21.0	2/2	28.2	0/2	–
	No drug		2/2	21.2	2/2	22.6	2/2	20.2
	100	C. glabrata	2/2	20.0	2/2	33.0	2/2	21.4
	50		2/2	19.5	2/2	32.8	2/2	19.4
	25		2/2	19.4	2/2	31.6	2/2	19.2
	No drug		2/2	17.2	2/2	30.3	2/2	18.1
	100	C. krusei	2/2	22.5	2/2	32.4	0/2	–
	50		2/2	22.2	2/2	32.1	2/2	69.1
	25		2/2	22.1	2/2	31.3	2/2	58.2
	No drug		2/2	21.8	2/2	28.5	2/2	22.9
	100	C. parapsilosis	2/2	39.4	2/2	36.6	0/2	–
	50		2/2	38.3	2/2	36.2	0/2	–
	25		2/2	38.2	2/2	32.2	0/2	–
	No drug		2/2	37.9	2/2	30.7	2/2	31.8
	100	C. tropicalis	2/2	23.2	2/2	24.6	2/2	54.8
	50		2/2	22.8	2/2	24.2	2/2	44.8
	25		2/2	22.5	2/2	24.1	2/2	39.5
	No drug		2/2	21.2	2/2	21.0	2/2	19.7
Voriconazole	100	C. auris	2/2	21.4	2/2	21.1	2/2	26.8
	50		2/2	21.3	2/2	21.0	2/2	21.8
	25		2/2	21.2	2/2	20.7	2/2	21.0
	No drug		2/2	21.2	2/2	20.4	2/2	20.3
	100	C. albicans	2/2	25.4	2/2	28.1	2/2	108.4
	50		2/2	20.9	2/2	23.8	2/2	99.2
	25		2/2	20.8	2/2	23.4	2/2	85.7
	No drug		2/2	20.6	2/2	23.4	2/2	20.1
	100	C. glabrata	2/2	19.9	2/2	37.1	2/2	22.7
	50		2/2	18.0	2/2	33.3	2/2	21.9
	25		2/2	17.9	2/2	32.5	2/2	20.9
	No drug		2/2	17.9	2/2	31.7	2/2	17.2
	100	C. krusei	2/2	24.3	2/2	28.6	2/2	61.8
	50		2/2	22.6	2/2	27.9	2/2	34.8
	25		2/2	22.5	2/2	26.6	2/2	34.3
	No drug		2/2	22.2	2/2	26.5	2/2	22.9
	100	C. parapsilosis	2/2	37.5	2/2	37.3	0/2	–
	50		2/2	37.3	2/2	32.5	0/2	–
	25		2/2	37.3	2/2	30.2	0/2	–
	No drug		2/2	36.1	2/2	31.6	2/2	32.7
	100	C. tropicalis	2/2	22.4	2/2	21.7	2/2	55.8
	50		2/2	22.3	2/2	21.7	2/2	42.8
	25		2/2	22.2	2/2	21.7	2/2	41.1
	No drug		2/2	19.6	2/2	19.9	2/2	19.2

^aCandida species were allowed to grow in the presence of azole antifungal agents in resin containing (BacT/Alert FAP and Bactec PAF) or non-containing (Bactec MICF) blood culture bottles, following bottles’ incubation in the BacT/Alert VIRTUO or the Bactec FX blood culture automated system, respectively. In the case of recovery, the times to detection were recorded for each of the Candida species used in the study. The symbol – indicates no recovery.

^bSee text and Table S1 for details.