FIG 1.
Evaluation of viral RNA recovery for PV positive stool samples by 11 extraction methods. (A and B) Box plots of cycle threshold (CT) value (A) and viral genome copies/μL (B) of the PV stool samples according to each extraction method. Extracted RNA was tested by RT-qPCR assay using PV specific primers and probe. Each gray circle represents a sample. Each stool sample was tested in the RT-qPCR in duplicates run in parallel with Sabin 1 standards. The box plots show the mean (represented by red dot), median, lower quartile, and upper quartile of the data points for samples extracted by each method, and the whiskers show the range. P values are based on a linear mixed effects regression analysis.