TABLE 1.
Summary of RNA concentration and integrity for all samples recovered by the 11 extraction methods
| Extraction | Total stool samples tested | PV strains contained in samplesa | Percentage of samples with RNA concn | Mean RNA concn ± SD (ng/μL) | Percentage of samples with RINe | Mean RINe ± SD |
|---|---|---|---|---|---|---|
| AllPrep PowerFecal DNA/RNA kit | 28 | SL1 and SL3 | 100 (28/28) | 2.61 ± 7.06 | 100 (28/28) | 2.27 ± 0.54 |
| High Pure viral RNA kit without proteinase K (Roche protocol A) | 28 | SL1 and SL3 | 100 (28/28) | 1.65 ± 2.74 | 100 (28/28) | 1.68 ± 0.39 |
| High Pure viral RNA kit with proteinase K (Roche protocol B) | 31 | PV2, SL1, and SL3 | 100 (31/31) | 2.07 ± 3.71 | 83.87 (26/31) | 2.3 ± 0.60 |
| KingFisher MagMAX viral RNA isolation kit | 28 | SL1 and SL3 | 100 (28/28) | 1.54 ± 3.60 | 100 (28/28) | 3.55 ± 1.66 |
| KingFisher MagMAX pathogen RNA/DNA kit (SV) | 28 | SL1 and SL3 | 100 (28/28) | 1.66 ± 2.71 | 100 (28/28) | 3.43 ± 1.86 |
| KingFisher MagMAX pathogen RNA/DNA kit (MV) | 28 | SL1 and SL3 | 100 (28/28) | 2.22 ± 4.87 | 100 (28/28) | 3.78 ± 1.93 |
| KingFisher MagMAX pathogen RNA/DNA kit (LV) | 16 | SL1 and SL3 | 100 (16/16) | 3.10 ± 5.47 | 100 (16/16) | 2.56 ± 1.31 |
| MagMAX viral RNA isolation kit (manual) | 58 | PV2, SL1, and SL3 | 100 (58/58) | 3.30 ± 4.51 | 89.8 (53/58) | 2.90 ± 1.39 |
| QIAamp viral RNA minikit | 59 | PV2, SL1, and SL3 | 89.8 (53/59) | 2.73 ± 8.20 | 84.7 (50/59) | 2.19 ± 0.84 |
| Zymo quick viral RNA kit (protocol A) | 28 | SL1 and SL3 | 100 (28/28) | 2.72 ± 9.45 | 100 (28/28) | 2.58 ± 0.96 |
| Zymo quick viral RNA kit (protocol B) | 31 | PV2, SL1, and SL3 | 45.2 (14/31) | 1.62 ± 2.65 | 32.3 (10/31) | 1.9 ± 0.57 |
a
PV2, Sabin-like type 2 poliovirus; SL1, Sabin-like 1 poliovirus; SL3, Sabin-like 3 poliovirus.