TABLE 2.
Characteristics of the extraction methods
| Extraction methoda | Sample input (μL) | Total sample/lysis vol before loading the column/ bead capture (μL) | Sample output (μL) | Concentration factor | Chemistry | Process | Lab |
|---|---|---|---|---|---|---|---|
| AllPrep PowerFecal DNA/RNA kit | 200 | 3,400 | 50 | 4 | Spin column | Manual | ICL |
| High Pure viral RNA kit without proteinase K (Roche protocol A) | 200 | 600 | 50 | 4 | Spin column | Manual | ICL |
| High Pure viral RNA kit with proteinase K (Roche protocol B) | 200 | 600 | 50 | 4 | Spin column | Manual | NIBSC |
| KingFisher MagMAX viral RNA isolation kit | 300 | 900 | 50 | 6 | Magnetic beads | Semiautomated | ICL |
| KingFisher MagMAX pathogen RNA/DNA kit (SV) | 140 | 940 | 50 | 2.8 | Magnetic beads | Semiautomated | ICL |
| KingFisher MagMAX pathogen RNA/DNA kit (MV) | 300 | 920 | 50 | 6 | Magnetic beads | Semiautomated | ICL |
| KingFisher MagMAX pathogen RNA/DNA kit (LV) | 700 | 4,619 | 90 | 7.8 | Magnetic beads | Semiautomated | ICL |
| MagMAX viral RNA isolation kit (manual) | 400 | 1,200 | 50 | 8 | Magnetic beads | Manual | ICL and NIBSC |
| QIAamp viral RNA minikit | 560 | 5,040 | 50 | 11.2 | Spin column | Manual | ICL and NIBSC |
| Zymo quick RNA viral kit (protocol A) | 800 | 3,200 | 50 | 16 | Spin column | Manual | ICL |
| Zymo quick RNA viral kit (protocol B) | 400 | 1,600 | 25 | 16 | Spin column | Manual | NIBSC |
a
Methods starting with KingFisher were extracted automatically using the KingFisher Duo instrument. For the KingFisher MagMAX pathogen RNA/DNA kits, SV indicates small volume, MV indicates medium volume, and LV indicates large volume.