FIG 4.

Interferon gamma signaling induces transcription factor binding and epigenetic modifications upstream of HERV-K102. (A) Visualization of chromatin immunoprecipitation (ChIP)-sequencing results for STAT1 binding, IRF1 binding, and H3K27ac prior to and following IFN-γ treatment for 24 h in primary MDMs. (B) Close-up visualization of LTR12F and STAT1 or IRF1 binding. Three arrows are depicted for IRF1 binding analysis, with each arrow representing a potential IRF-binding element (IRF-E) sequence motif within LTR12F. (C) Visualization of ATAC-seq (Assay for Transposase-Accessible Chromatin using Sequencing) and ChIP-sequencing for H3K4me2, H3K27ac, and H3K79me2 prior to and following IFN-γ treatment for 24 h in HeLa cells. Peak locations, fold-enrichment values, and P values are shown in Fig. S4.