FIG 7.

In some larvae, voriconazole eliminates persister isolates less efficiently than nonpersister strains in a Galleria mellonella infection model. The fungal burden was measured by qPCR 72 h after infection with 10⁴ conidia (A) or at 48 h after infection with 5 × 10⁴ conidia (B) for all strains; the fungal burden was greatly lower in larvae that had received a voriconazole treatment (8 μg/mL) than in those who had not. The reduction in burden was bigger in nonpersister isolates than in persister isolates, although these differences were not significant. Several treated larvae infected with persister isolates had noticeably higher burdens than others: 4/11 for PD-9 and 3/11 for PD-104 (A) and 3/11 for PD-9 and 4/11 for PD-104 (B). Each graph displays the combined data from two independent experiments. Persister isolates are labeled with an asterisk (*).