TABLE 1.
Primer sequences used in this studya
| Method | Forward primer sequence | Reverse primer sequence |
|---|---|---|
| Initial amplicon generation | TTTCTGTTGGTGCTGATATTGCctggtgacgcataccgaacg | ACTTGCCTGTCGCTCTATCTTCacctcggtcagattgcgagg |
| Splint | /5Phos/GAACGACATGGCTACGATTTCTGTTGGTGCTGATATTGC | TGTGAGCCAAGGAGTTGACTTGCCTGTCGCTCTATCTTC |
| DumBell | /5Phos/TTTCTGTTGGTGCTGATATTGC | /5Phos/ACTTGCCTGTCGCTCTATCTTC |
| uDumBell | /5Phos/GUCTATTTTCTGTTGGTGCTGATATTGC | /5Phos/GUCTATACTTGCCTGTCGCTCTATCTTC |
| Gibson | GGCGGCGACCTGTCGTTTCTGTTGGTGCTGATATTGC | CGACAGGTCGCCGCCACTTGCCTGTCGCTCTATCTTC |
| Restriction enzyme cloning | AAAATCTAGATTTCTGTTGGTGCTGATATTGC | AAAAAGATCTACTTGCCTGTCGCTCTATCTTC |
| TelN protelomerase | TATCAGCACACAATTGCCCATTATACGCGCGTATAATGGACTATTGTGTGCTGATATTTCTGTTGGTGCTGATATTGC | ATAGTCGTGTGTTATCAGGTAATATGCGCGCATATTACCCGTTAACACACGACTATACTTGCCTGTCGCTCTATCTTC |
| easyDB | GGCGTCTCAAAACGCCCGT *T*T*T*C*T* GTTGGTGCTGATATTGC | GGCGTCTCAAAACGCCCGT*A*C*T*T*GCCTGTCGCTCTATCTTC |
a
An asterisk between nucleotides denotes a phosphorothioate bond. Lowercase bases indicate a match to the target gene (rv0678); the underlined bases are restriction enzyme cut sites, while the bold indicate complementarity to the initial amplicon.