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F181 and A184 of STM1344 are important for FlhD binding. (A) Interface between STM1344 and FlhD. STM1344 residues in the interface are shown as a stick model. (B) Pulldown of native and mutant STM1344 by FlhD4C2 with the His tag. The two mutated residues (F181 and A184) of STM1344 lose interaction with FlhD4C2.
