FIG 2.

Identification of DUBs in L. longbeachae. (A) Diagram of the strategies used to identify DUBs from L. longbeachae. (B) Lysates of L. longbeachae were incubated with HA-Ub-VME for 2 h at 37°C. Following immunoprecipitation (IP) with anti-HA agarose, HA-Ub-VME-reactive proteins were probed by Western blotting with an HA-specific antibody. Ten percent and 40% indicate the amount of anti-HA IP products loaded on the gel. (C) Predicted DUB domain of the candidate DUBs identified by bioinformatics analysis using HHpred. Results in panel B are one representative from three independent experiments with similar results.