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Cleavage of diubiquitin by putative L. longbeachae DUBs. A panel of diubiquitins with various linkage types was incubated with purified LLO_1014ΔTM (A), LLO_2238 (B), LLO_2491ΔC102 (C), LLO_33911009–1200 (D), or LLO_2066 (E) at 37°C for 2 h. Protein samples separated by SDS-PAGE were detected by Coomassie brilliant blue (CBB) staining. Data shown are one representative from three independent experiments.
