FIG 8.

L. longbeachae OTU family DUBs regulate the association of polyubiquitinated proteins with their phagosome. (A and B) U937 cells infected with indicated L. longbeachae strains for 2 h (MOI = 10) were fixed infection samples and were stained with L. longbeachae and FK1 (A) or K63-linkage-specific (B) polyubiquitin antibodies to detect ubiquitinated proteins on the bacterial phagosome. The proportion of LCVs positively stained with ubiquitin antibodies is shown. At least 100 phagosomes were counted for each sample, and data are presented as mean ± SD scored from three coverslips. Similar results are obtained from three independent experiments. Statistics analysis was conducted via unpaired two-tailed Student t tests, and a P value of <0.05 indicates a significant difference.