FIG 2.

Progress curves of CMY-2 (blue), CMY-172 (orange), and CMY-178 (red) and no-enzyme control (green) for ceftazidime hydrolysis. All reactions were performed with 200 nM enzyme and 75 μM ceftazidime. Hydrolysis of ceftazidime results in a loss of absorbance at 260 nm.