TABLE 1.

MICs and β-lactamase genes of the strains described in this study^a

Strain	Description	β-Lactamase gene	MICs (μg/mL) of:
			MEM	IMP	PIP	TZP	FOS	AMK	LEV	ATM	CTX	FEP	CXM	CAZ	CZA4	CZA8	CFDC
AR13438	Clinical E. coli isolate	blaCMY-178, blaTEM-1, blaCTX-M-27	0.125	1	>256	64/4	1	>256	32	32	>128	128	>1,024	2,048	64/4	32/8	0.25
KPCZA02	Clinical K. pneumoniae isolate	blaKPC-2, blaCMY-172, blaTEM-1B, blaCTX-M-65	128	64	>256	>256/4	>1,024	>256	32	>128	>128	>128	>1,024	2,048	128/4	64/8	1
E. coli DH5α			0.03	0.25	1	0.5/4	0.25	1	0.03	0.06	0.03	0.06	4	0.25	0.06/4	0.06/8	<0.03
E. coli DH5α-pCR2.1			0.03	0.25	1	0.5/4	0.25	1	0.03	0.12	0.06	0.12	4	0.25	0.12/4	0.12/8	<0.03
E. coli DH5α-pCR2.1-CMY-2	Transformant of the recombinant plasmid containing blaCMY-2	bla CMY-2	0.03	0.25	256	64/4	0.25	1	0.06	32	32	2	128	256	0.5/4		0.125
E. coli DH5α-pCR2.1-CMY-178	Transformant of the recombinant plasmid containing blaCMY-178	bla CMY-178	0.06	0.25	256	128/4	0.25	1	0.06	32	128	64	1,024	>2,048	64/4	32/8	1
E. coli DH5α-pCR2.1-CMY-172	Transformant of the recombinant plasmid containing blaCMY-172	bla CMY-172	0.06	0.25	256	128/4	0.25	2	0.03	8	32	16	64	512	16/4	2/8	1
E. coli J53			0.03	0.5	4	2/4	0.5	2	0.03	0.12	0.06	0.06	16	0.25	0.25/4	/b	/
E. coli J53-AR13438	Conjugant containing plasmid pAR13438_2	bla CMY-178	0.06	0.5	64	16/4	0.5	2	0.03	32	32	32	256	1024	32/4	/	/
E. coli J53-KPCZA02	Conjugant containing plasmid pKPCZA02_4	bla CMY-172	0.06	0.5	64	16/4	0.5	2	0.03	16	16	32	256	512	16/4	/	/

^aMEM, meropenem; IMP, imipenem; PIP, piperacillin; TZP, piperacillin-tazobactam; FOS, fosfomycin; AMK, amikacin; LEV, levofloxacin; ATM, aztreonam; CTX, cefotaxime; FEP, cefepime; CXM, cefuroxime; CAZ, ceftazidime; CZA4, avibactam was tested at a fixed concentration of 4 μg/mL in combination with double dilutions of ceftazidime; CZA8, avibactam was tested at a fixed concentration of 8 μg/mL in combination with double dilutions of ceftazidime.

^b/, no relevant measurements were taken.