TABLE 1.
Mass spectrometry conditionsa for determination of fecal antibiotic concentrations
| Analyte | Q1 (m/z) | Q3 (m/z) |
|---|---|---|
| Vancomycin (T1) | 724.900 | 144.000 |
| Vancomycin (T2) | 724.900 | 1143.500 |
| Fidaxomicin (T1) | 1079.500 | 847.600 |
| Fidaxomicin (T2) | 1079.500 | 617.200 |
| Op1118 (T1) | 1009.500 | 777.500 |
| Op1118-T2 | 1009.500 | 505.000 |
| Codeine ISb (T1) | 300.100 | 152.000 |
| Methadone ISb (T1) | 310.200 | 265.200 |
a
Mass transitions (in m/z) are shown for each compound run. Mass transitions are tandem MS (MS/MS) gas-phase dissociation reactions wherein the ionized parent molecule is selected in the first quadrupole (Q1), subjected to collisions with gas to affect molecule fragmentation and fragmentation products are chosen in the final quadrupole (Q3). Two mass transitions are chosen: (1) Quantifier (T1) for quantitation via peak area and (2) Qualifier (T2) for confirmation of the molecule and MS/MS experiment by comparing height ratios of T1 and T2.
b
Internal standard.