TABLE 2.
Distribution of polymyxin resistance-related gene mutations among 101 polymyxin- and carbapenem-resistant K. pneumoniae isolatesa,b
| Gene (n1) | Gene mutation (+, −/n2) |
|---|---|
| crrA(0) | V167I (−/1), K228R (−/2), D198N (−/2), deletion (−/43) |
| crrB(1) | 1−249 aa del (−/43), C68S (−/20), Q301K (−/2), I27V (−/2), I66V (−/2), Q239H (−/2), T76A (−/2), V193G (+/1), T276A (−/1), Q287K (−/1), M63I (−/1) |
| crrC(2) | 1−22 aa del (−/43), A15V (−/4), V84L (−/3), V82I (−/1), P68T (+/1), 107−129 aa del (+/1) |
| pmrA(2) | A41T (−/4), M66I (−/3), I220N (−/2), D221E (−/2), G53V (+/1), D86E (+/1), E57G (−/1) |
| pmrB(4) | T246A (−/71), R256G (+/51), L213M (−/7), P344L (+/3), G358A (−/3), K220Q (−/3), S363I (−/3), T157P (+/1), M11I (−/1), G345E (−/1) |
| pmrC(4) | I138V (−/92), Q319R (−/81), C27F (−/72), D249E (−/6), L39V (−/3), T224K (−/3), V36I (−/3), P287S (+/2), D75E (−/2), S257L (+/1), A223S (+/1), E408K (−/1), K509T (−/1), I142V (−/1), A162V (−/1) |
| pmrD(0) | T60M (−/8), G80D (−/2) |
| phoP(2) | D191A (+/2), E82D (−/1) |
| phoQ(4) | P424L (+/3), G150D (−/2), D438E (+/1), A145S (−/1), T156S (−/1) |
| mgrB(27) | C28R (+/1), M1V (+/1), I45F (+/1), T-35C (+/2), deletion (+/1), IS903 (+/4), ISkpn14 (+/9), ISkpn18 (+/2), ISkpn26 (+/2), disrupt (+/3), 7Vfs (+/1) |
| arnA(0) | T450N (−/8), L260I (−/5), S18A (−/4), K442N (−/2), A22V (−/1), S061T (−/1), N660H (−/1) |
| arnB(7) | D101A (−/90), T272A (−/5), D274E (−/5), A148V (−/3), A124T (+/1), R269C (+/1), S371F (+/1), G367V (+/1), P264S (+/2), G36D (+/1), I115V (−/1), A261T (−/1), V308A (−/1), I89V (−/1) |
| arnC(1) | T30S (−/2), A36T (−/1), E204V (+/1), I286V (−/1) |
| arnD(2) | L94I (−/20), V300I (−/15), I53V (−/14), S271C (+/2), V187T (−/2), D209E (−/1), V247E (−/1), I300V (−/1), S164P (−/1), S188F (−/1), A225P (−/1) |
| arnE(0) | |
| arnF(3) | A45P (−/8), P100L (+/3), G46S (−/1), G89A (−/1) |
| arnT(3) | H156Q (−/12), I117V (−/11), D441E (−/5), G164S (+/2), S404D (−/2), T153A (−/2), S157R (−/2), K372R (−/2), S404C (+/1), S404S (−/1), K337N (−/1), A153V (−/1), V299M (−/1) |
| acrA(0) | A188T (−/74) |
| acrB(0) | R716L (−/68), E639D (−/4) |
| acrR(71) | ISkpn26 (+/67), L34F (+/2), 81Ffs (+/1), 121Gfs (+/1) |
| kpnE(1) | G67V (+/12), K112Q (−/7), A55V (+/1) |
| kpnF(0) | |
| ramA(0) | Deletion (−/10) |
| ramR(11) | Q122c (+/2), A6V (−/8), L66del (+/2), D77H (−/2), H186N (−/2), V67del (−/2), R3H (+/1), A159T (+/1), 1−38 aa del (+/1), E165K (+/1), G151D (+/1), L111del (+/1), S112R (+/1), A20T (−/1), I141T (−/1), A19V (−/1) |
| LpxM(0) | S253G (−/80), T229S (+/69), N7I (+/32), N6K (−/3), E76D (−/1), deletion (−/10) |
| yciM(2) | N212T (+/83), N212S (−/4), A265V (−/3), V163M (−/3), R350C (+/2), A231T (−/1), V298I (−/1) |
| soxS(0) | I26V (−/10), R29K (−/2) |
| soxR(0) | D142E (−/10), E143D (−/2) |
a
Gene mutations, involving genes deleted, stopped, truncated, frameshifted, or inserted by an insertion sequence.
b
n1, the number of deleterious mutations of the corresponding genes in the current study; n2, the number of strains with a gene variation; +, the mutation was predicted as the deleterious mutation; −, the mutation was predicted as the neutral mutation; fs, frameshift mutation.
c
Stop codon. Del, deletion. The bold variation shows the deleterious variation defined in this study.