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Primers used for PCR amplification of HCMV mRNAsa
| Primer name | Nucleotide sequence (5′ to 3′) |
|---|---|
| IE1 forward | CAAGAGAAAGATGGACCCTG |
| IE1 reverse | CGAGTTCTGCCAGGACATC |
| LUNA round 1 forward | ATGACCTCTCCTCCACACC |
| LUNA round 1 reverse | GACGCTATATTTAGGGCTTCC |
| LUNA round 2 forward | GAGCCTTGACGACTTGGTAC |
| LUNA round 2 reverse | GGAAAAACACGCGGGGGA |
| GAPDH forward | GAGTCAACGGATTTGGTCGT |
| GAPDH reverse | TTGATTTTGGAGGGATCTCG |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LUNA, latency unique nuclear antigen.
