TABLE 2.

Missense mutations in OprD in 29 isolates of the validation data set and associated meropenem susceptibility

Isolate	Meropenem susceptibility	OprD reference variant	Missense mutation(s)	Predicted functional effect (SNAP2)	Expected prediction accuracy (SNAP2 [%])
287.12499_PA	Intermediate	OprD_3	R154H	Effect	95
287.12621_PA	Intermediate	OprD_1	L359P	Effect	75
287.1101_PA	Intermediate	OprD_2	L434R (in L812)	Effect	80
287.5955_PA	Resistant	OprD_11	L127V	Neutral	93
287.12587_PA	Resistant	OprD_4	R154C	Effect	95
287.8031_PA	Resistant	OprD_5	S278P	Effect	91
287.7799_PA	Resistant	OprD_1	S278P	Effect	85
287.1095_PA	Resistant	OprD_5	S278P	Effect	91
287.12616_PA	Resistant	OprD_1	H288R	Effect	85
287.12490_PA	Resistant	OprD_1	L292Q	Effect	80
287.5956_PA	Resistant	OprD_1	G307D	Neutral	57
287.8490_PA	Resistant	OprD_6	G316D	Neutral	61
287.12754_PA	Resistant	OprD_1	G316D	Effect	71
287.12755_PA	Resistant	OprD_1	G316D	Effect	71
287.12669_PA	Resistant	OprD_1	Q340P	Effect	80
287.5971_PA	Resistant	OprD_1	G383D (in L810)	Effect	80
287.12734_PA	Resistant	OprD_2	S403P (in L812)	Effect	63
287.12639_PA	Resistant	OprD_6	L409P (in L812)	Effect	76
287.860_PA	Susceptible	OprD_2	A8S	Neutral	97
502940-8-20	Susceptible	OprD_1	K34N	Neutral	82
502940-9-20	Susceptible	OprD_1	K34N	Neutral	82
502940-3-20	Susceptible	OprD_1	K34N, A247S	Neutral (each)	82, 97
287.7801_PA	Susceptible	OprD_4	D43N	Neutral	95
501539-19	Susceptible	OprD_5	V63A	Neutral	93
500658-19	Susceptible	OprD_6	T66S	Neutral	97
721748-19	Susceptible	OprD_6	T66S	Neutral	97
287.1066_PA	Susceptible	OprD_1	V86I	Neutral	93
287.1043_PA	Susceptible	OprD_2	G98A, V129I	Neutral (each)	82, 97
287.1190_PA	Susceptible	OprD_2	V129I	Neutral	97